LOCUS       V004341                 3772 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004341
VERSION     V004341
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Yamagishi J, Kojima T, Oyamada Y, Fujimoto K, Hattori H, Nakamura S,
            Inoue M.
  TITLE     Alterations in the DNA topoisomerase IV grlA gene responsible for
            quinolone resistance in Staphylococcus aureus
  JOURNAL   Antimicrob. Agents Chemother. 40 (5), 1157-1163 (1996)
   PUBMED   8723458
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Inoue R, Kaito C, Tanabe M, Kamura K, Akimitsu N, Sekimizu K.
  TITLE     Genetic identification of two distinct DNA polymerases, DnaE and
            PolC, that are essential for chromosomal DNA replication in
            Staphylococcus aureus
  JOURNAL   Mol. Genet. Genomics 266 (4), 564-571 (2001)
   PUBMED   11810227
REFERENCE   3  (bases 1 to 3772)
  AUTHORS   Matsuo M, Kurokawa K, Nishida S, Li Y, Takimura H, Kaito C, Fukuhara
            N, Maki H, Miura K, Murakami K, Sekimizu K.
  TITLE     Isolation and mutation site determination of the
            temperature-sensitive murB mutants of Staphylococcus aureus
  JOURNAL   FEMS Microbiol. Lett. 222 (1), 107-113 (2003)
   PUBMED   12757953
REFERENCE   4  (bases 1 to 3772)
  AUTHORS   Kurokawa K, Matsuo M, Sekimizu K.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-MAR-2005) Kenji Kurokawa, The University of Tokyo,
            Graduate School of Pharmaceutical Sciences; 7-3-1, Hongo, Bunkyo-ku,
            Tokyo 113-0033, Japan (E-mail:kurokawa@mol.f.u-tokyo.ac.jp,
            URL:http://www.f.u-tokyo.ac.jp/~hassei/, Tel:81-3-5841-4821,
            Fax:81-3-5684-2973)
REFERENCE   5  (bases 1 to 3772)
  TITLE     Direct Submission
REFERENCE   6  (bases 1 to 3772)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Antimicrob.
            Agents Chemother."; date: "1996"; volume: "40"; issue: "5"; pages:
            "1157-1163"
            SGRef: number: 2; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2001"; volume: "266"; issue: "4"; pages: "564-571"
            SGRef: number: 3; type: "Journal Article"; journalName: "FEMS
            Microbiol. Lett."; date: "2003"; volume: "222"; issue: "1"; pages:
            "107-113"
            SGRef: number: 4; type: "Journal Article"; journalName: "Submitted
            (03-MAR-2005) Kenji Kurokawa, The University of Tokyo, Graduate
            School of Pharmaceutical Sciences"; volume: " 7-3-1, Hongo,
            Bunkyo-ku, Tokyo 113-0033, Japan
            (E-mail:kurokawa@mol.f.u-tokyo.ac.jp,
            URL:http://www.f.u-tokyo.ac.jp/~hassei/, Tel:81-3-5841-4821, Fax";
            pages: "81-3-5684-2973"
            SGRef: number: 5; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3772
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..57
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     rep_origin      264..852
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1430..2077)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(2359..3360)
                     /label="repB"
                     /note="RepB replication protein"
ORIGIN
        1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc
       61 actggcggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
      121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
      181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
      241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
      301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
      361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
      421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
      481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
      541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
      601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
      661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
      721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
      781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
      841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
      901 tttgctcagg caaccacttc caagtaaagt ataacacact atactttata ttcataaagt
      961 gtgtgctctg cgaggctgtc ggcagtgccg accaaaacca taaaaccttt aagacctttc
     1021 ttttttttac gagaaaaaag aaacaaaaaa acctgccctc tgccacctca gcaaaggggg
     1081 gttttgctct cgtgctcgtt taaaaatcag caagggacag gtagtatttt ttgagaagat
     1141 cactcaaaaa atctccacct ttaaaccctt gccaattttt attttgtccg ttttgtctag
     1201 cttaccgaaa gccagactca gcaagaataa aatttttatt gtctttcggt tttctagtgt
     1261 aacggacaaa accactcaaa ataaaaaaga tacaagagag gtctctcgta tcttttattc
     1321 agcaatcgga tctggagctg taatataaaa accttcttca actaacgggg caggttagtg
     1381 acattagaaa accgactgta aaaagtacag tcggcattat ctcatattat aaaagccagt
     1441 cattaggcct atctgacaat tcctgaatag agttcataaa caatcctgca tgataaccat
     1501 cacaaacaga atgatgtacc tgtaaagata gcggtaaata tattgaatta cctttattaa
     1561 tgaattttcc tgctgtaata atgggtagaa ggtaattact attattattg atatttaagt
     1621 taaacccagt aaatgaagtc catggaataa tagaaagaga aaaagcattt tcaggtatag
     1681 gtgttttggg aaacaatttc cccgaaccat tatatttctc tacatcagaa aggtataaat
     1741 cataaaactc tttgaagtca ttctttacag gagtccaaat accagagaat gttttagata
     1801 caccatcaaa aattgtataa agtggctcta acttatccca ataacctaac tctccgtcgc
     1861 tattgtaacc agttctaaaa gctgtatttg agtttatcac ccttgtcact aagaaaataa
     1921 atgcagggta aaatttatat ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa
     1981 tttctgtggt tatactaaaa gtcgtttgtt ggttcaaata atgattaaat atctcttttc
     2041 tcttccaatt gtctaaatca attttattaa agttcatttg atatgcctcc taaattttta
     2101 tctaaagtga atttaggagg cttacttgtc tgctttcttc attagaatca atcctttttt
     2161 aaaagtcaat gctcgtcgta taacagatgc gatgatgcag accaatcaac atggcacctg
     2221 ccattgctac ctgtacagtc aaggatggta gaaatgttgt cggtcctatt aataactcgt
     2281 cttcctaagc atccttcaat ccttttaata acaattatag catctaatct tcaacaaact
     2341 ggcccgtttg ttgaactact ctttaataaa ataatttttc cgttcccaat tccacattgc
     2401 aataatagaa aatccatctt catcggcttt ttcgtcatca tctgtatgaa tcaaatcgcc
     2461 ttcttctgtg tcatcaaggt ttaatttttt atgtatttct tttaacaaac caccatagga
     2521 gattaacctt ttacggtgta aaccttcctc caaatcagac aaacgtttca aattcttttc
     2581 ttcatcatcg gtcataaaat ccgtatcctt tacaggatat tttgcagttt cgtcaattgc
     2641 cgattgtata tccgatttat atttattttt cggtcgaatc atttgaactt ttacatttgg
     2701 atcatagtct aatttcattg cctttttcca aaattgaatc cattgttttt gattcacgta
     2761 gttttctgta ttcttaaaat aagttggttc cacacatacc aatacatgca tgtgctgatt
     2821 ataagaatta tctttattat ttattgtcac ttccgttgca cgcataaaac caacaagatt
     2881 tttattaatt tttttatatt gcatcattcg gcgaaatcct tgagccatat ctgacaaact
     2941 cttatttaat tcttcgccat cataaacatt tttaactgtt aatgtgagaa acaaccaacg
     3001 aactgttggc ttttgtttaa taacttcagc aacaaccttt tgtgactgaa tgccatgttt
     3061 cattgctctc ctccagttgc acattggaca aagcctggat ttacaaaacc acactcgata
     3121 caactttctt tcgcctgttt cacgattttg tttatactct aatatttcag cacaatcttt
     3181 tactctttca gcctttttaa attcaagaat atgcagaagt tcaaagtaat caacattagc
     3241 gattttcttt tctctccatg gtctcacttt tccacttttt gtcttgtcca ctaaaaccct
     3301 tgatttttca tctgaataaa tgctactatt aggacacata atattaaaag aaacccccat
     3361 ctatttagtt atttgtttag tcacttataa ctttaacaga tggggttttt ctgtgcaacc
     3421 aattttaagg gttttcaata ctttaaaaca catacatacc aacacttcaa cgcacctttc
     3481 agcaactaaa ataaaaatga cgttatttct atatgtatca agataagaaa gaacaagttc
     3541 aaaaccatca aaaaaagaca ccttttcagg tgcttttttt attttataaa ctcattccct
     3601 gatctcgact tcgttctttt tttacctctc ggttatgagt tagttcaaat tcgttctttt
     3661 taggttctaa atcgtgtttt tcttggaatt gtgctgtttt atcctttacc ttgtctacaa
     3721 accccttaaa aacgttttta aaggctttta agccgtctgt acgttcctta ag
//
